Imaging:Article Title: Dynamic fibroblast–immune interactions shape recovery after brain injury
Article Snippet: Whole slide scanning (40×) was performed on an Aperio AT2 (Leica Biosystems). .. Primary antibodies used for mouse imaging include chicken anti-GFP (Aves Labs GFP-1020, 1:200), rabbit anti-dsRed (Takara 632496, 1:300), chicken anti-GFAP (Invitrogen PA1-10004, 1:200 or 1:500), rat anti-GFAP (2.2B10, Invitrogen 13-0300, 1:200), rat anti-ER-TR7 (Novus Biologicals NB100-64932, 1:200), rabbit anti-αSMA (Abcam ab5694, 1:300), rat anti-CD31 (MEC13.3, Biolegend 102514, 1:200), goat anti-Desmin (GenWay Biotech GWB-EV0472, 1:200), rat anti-PDGFRβ (APB5, Invitrogen 14-1402-82, 1:500), rabbit anti-NG2 (Millipore Sigma ab5320, 1:500), goat anti-Decorin (Novus Biologicals AF1060, 1:200), goat anti-collagen 1 (Southern Biotech 1310-01, 1:200 or 1:500), rabbit anti-collagen 6α1 (Novus Biologicals NB120-6588, 1:200), rat anti-periostin (345613, Novus Biologicals MAB3548, 1:200), rat anti-ICAM1 (YN1/1.7.4, Biolegend 116110, 1:200), Syrian hamster-anti-CD3ε (500A2, BD Biosciences 553238, 1:200), goat anti-S100A8 (R&D Systems AF3059, 1:200), chicken anti-NeuN (Millipore Sigma ABN91, 1:200), rabbit anti-IBA1 (Aif3, Fujifilm Wako 019-19741, 1:200–1:1,000), mouse anti-FGF13 (N235/22, Invitrogen MA5-27705, 1:100), goat anti-CD80 (R&D Systems AF740, 1:200), goat anti-ALPL (Novus Biologicals AF2910, 1:50), rabbit anti-LAMA1 (EPR27258-37, Abcam ab307542, 1:200), rabbit anti-SEMA3C (Invitrogen PA5-103168, 1:100), rabbit anti-CDH18 (Invitrogen PA5-112902, 1:50), rabbit anti-ALDH1A2 (Novus Biologicals NBP2-92915, 1:200), goat anti-SOX10 (R&D Systems AF2864, 1:300), rabbit anti-ASPA (Genetex GTX113389, 1:1,000), mouse anti-E-cadherin (Clone 36, BD Biosciences 610181), rat anti-I-A/I-E (MHCII, M5/114.15.2, eBioscience 14-5321-82), mouse anti-Ly76 (TER119, Biolegend 116232, 1:200), goat anti-mouse IgM (Invitrogen 31172, 1:200), and rabbit anti-cCasp3 (Cell Signaling Technology 9661T, 1:400; Cell Signaling Technology 9991, Histowiz). .. For marmoset imaging, rabbit anti-COL6 (Abcam ab6588, 1:500) was used.
Article Title: Dynamic fibroblast-immune interactions shape wound healing after brain injury
Article Snippet: .. Primary antibodies used for murine imaging include chicken anti-GFP (Aves Labs GFP-1020, 1:200), rabbit anti-dsRed (Takara 632496, 1:300), chicken anti-GFAP (Invitrogen PA1-10004, 1:200), rat anti-GFAP (2.2B10, Invitrogen 13-0300, 1:200), rat anti-ER-TR7 (Novus Biologicals NB100-64932, 1:200), rabbit anti- α SMA (Abcam ab5694, 1:300) rat anti-CD31 (MEC13.3, Biolegend 102514, 1:200), goat anti-Desmin (GenWay Biotech GWB-EV0472, 1:200), goat anti-Decorin (Novus Biologicals AF1060, 1:200), rabbit anti-Collagen 6 α 1 (Novus Biologicals NB120-6588, 1:200), rat anti-Periostin (345613, Novus Biologicals MAB3548, 1:200), rat anti-ICAM1 (YN1/1.7.4, Biolegend 116110, 1:200), syrian hamster-anti-CD3 ε (500A2, BD Biosciences 553238, 1:200), goat anti-S100A8 (R&D Systems AF3059, 1:200), chicken anti-NeuN (Millipore Sigma ABN91, 1:200), rabbit anti-Iba1 (Aif3, Fujifilm Wako 019-19741, 1:200-1:1000). .. For marmoset imaging, rabbit anti-COL6 (Abcam ab6588, 1:500) was used.
Article Title: Dynamic fibroblast-immune interactions shape recovery after brain injury.
Article Snippet: .. Materials & experimental systems n/a Involved in the study Antibodies Eukaryotic cell lines Palaeontology and archaeology Animals and other organisms Clinical data Dual use research of concern Plants Methods n/a Involved in the study ChIP-seq Flow cytometry MRI-based neuroimaging Antibodies Antibodies used Primary antibodies used for murine imaging include chicken anti-GFP (Aves Labs GFP-1020, 1:200), rabbit anti-dsRed (Takara 632496, 1:300), chicken anti-GFAP (Invitrogen PA1-10004, 1:200 or 1:500), rat anti-GFAP (2.2B10, Invitrogen 13-0300, 1:200), rat anti-ER-TR7 (Novus Biologicals NB100-64932, 1:200), rabbit anti-aSMA (Abcam ab5694, 1:300), rat anti-CD31 (MEC13.3, Biolegend 102514, 1:200), goat anti-Desmin (GenWay Biotech GWB-EV0472, 1:200), rat anti-PDGFRb (APB5, Invitrogen 14-1402-82, 1:500), rabbit antiNG2 (Millipore Sigma ab5320, 1:200), goat anti-Decorin (Novus Biologicals AF1060, 1:200), goat anti-Collagen 1 (Southern Biotech 1310-01, 1:500), rabbit anti-Collagen 6a1 (Novus Biologicals NB120-6588, 1:200 or 1:500), rat anti-Periostin (345613, Novus Biologicals MAB3548, 1:200), rat anti-ICAM1 (YN1/1.7.4, Biolegend 116110, 1:200), syrian hamster-anti-CD3e (500A2, BD Biosciences 553238, 1:200), goat anti-S100A8 (R&D Systems AF3059, 1:200), chicken anti-NeuN (Millipore Sigma ABN91, 1:200), rabbit anti-Iba1 (Aif3, Fujifilm Wako 019-19741, 1:200-1:1000), mouse anti-FGF13 (N235/22, Invitrogen MA5-27705, 1:100), goat anti-CD80 (R&D Systems AF740, 1:200), goat anti-ALPL (Novus Biologicals AF2910, 1:50), rabbit anti-LAMA1 (EPR27258-37, Abcam ab307542, 1:200), rabbit anti-SEMA3C (Invitrogen PA5-103168, 1:100), rabbit anti-CDH18 (Invitrogen PA5-112902, 1:50), rabbit anti-ALDH1A2 (Novus Biologicals NBP2-92915, 1:200), goat anti-SOX10 (R&D Systems AF2864, 1:300), rabbit anti-ASPA (Genetex GTX113389, 1:1000), mouse anti-E-Cadherin (Clone 36, BD Biosciences 610181), rat anti-I-A/I-E (MHCII, M5/114.15.2, eBioscience 14-5321-82), mouse anti-Ly76 (TER119, Biolegend 116232, 1:200), goat anti-mouse IgM (Invitrogen 31172, 1:200), and rabbit anti cleaved Caspase 3 (Cell Signaling Technology 9661T, 1:400; Cell Signaling Technology 9991, Histowiz). ..
other:
Staining:Article Title: Identification of functional heterogeneity of immune cells and tubular-immune cellular interplay action in diabetic kidney disease
Article Snippet: The urine albumin concentration was measured using the ELISA kit (Bethyl Laboratories, Montgomery, TX, USA). .. The antibodies and reagents used in this study included rabbit anti-CD206 (ab64693, 1:400, Abcam, Cambridge, UK), goat anti-S100a8 (AF3059, 1:100, R&D systems, MN, USA), goat anti-S100a9 (1:100, AF2065, R&D systems, MN, USA), DNA was stained with Hoechst 33342 (1:1000, H3570; Thermo Fisher Scientific, CA, USA), dapagliflozin (3 mg/kg/d, S1548, Selleck, TX, USA) ..
Immunohistochemistry:Article Title: Bacterial meningitis in the early postnatal mouse studied at single-cell resolution
Article Snippet: .. The following antibodies were used for tissue immunohistochemistry and immunoblotting: goat anti-CD45 (R&D Systems AF114-SP); rat anti-CD206/MRC1 (Bio-Rad MCA2235T); goat anti-CD206 (R&D Systems AP2535); rat anti-LYVE1 (Thermo Fisher/eBioscience 14-0443-82); rat anti-PU.1/Spi-1 (Novus Biologicals MAB7124); rat anti-CD180/RP105 antibody, PE (eBioscience 12-1801-81); goat anti-S100A8 (R&D Systems AF3059); rabbit anti-ERG (Cell Signaling Technologies 97249); rat anti-PECAM1/CD31 (BD Biosciences 553370); mouse anti-CLDN5, Alexa Fluor 488 conjugate (Invitrogen 352588); mouse anti-CLDN5 (Invitrogen 35-2500); rabbit anti-ZO1 (Invitrogen 40-2200); rabbit anti-LEF1 rabbit (Cell Signaling Technologies 2230); rabbit anti-LEF1, Alexa Fluor 647 conjugate (Cell Signaling Technologies 14022); rabbit anti-Occludin (Invitrogen 406100); goat anti-VEGFR2/KDR (R&D Systems AF644-SP); sheep anti-FOXP2 (R&D Systems AF5647-SP); rabbit anti-SATB2 (Abcam ab92446); rat anti-IL-6 (Biolegend 504501); rabbit anti-COL14A1 (Novus Biologicals NBP2-15940); chicken anti-GFP (Abcam Ab13970), rat anti-HA (Proteintech 7c9); rabbit anti-HA (homemade); goat anti-E-cadherin (R&D Systems AF748); rabbit anti-E-cadherin (Cell Signaling Technologies 3195); rabbit anti-AIFM3 (Novus Biologicals NBP1-76889); rabbit anti-COL25A1 (G-Biosciences ITT1021); goat anti-IGSF8 (R&D systems AF3117-SP); rabbit anti-NNAT (Abcam ab27266); mouse anti-GAPDH (Cell Signaling Technologies 97166S); rabbit anti-GAPDH (Cell Signaling Technologies 5174S); Streptavidin, Alexa Fluor 488 conjugate (Invitrogen S11223); Streptavidin, Alexa Fluor 647 conjugate (Invitrogen S32357). .. Alexa-Fluor-conjugated secondary antibodies were from Invitrogen.
Article Title: Bacterial meningitis in the early postnatal mouse studied at single-cell resolution
Article Snippet: .. The following antibodies were used for tissue immunohistochemistry and immunoblotting: goat anti-CD45 (R&D Systems AF114-SP); rat anti-CD206/MRC1 (Bio-Rad MCA2235T); goat anti-CD206 (R&D Systems AP2535); rat anti-LYVE1 (Thermo Fisher/eBioscience 14-0443-82); rat anti-PU.1/Spi-1 (Novus Biologicals MAB7124); rat anti-CD180/RP105 antibody, PE (eBioscience 12-1801-81); goat anti-S100A8 (R&D Systems AF3059); rabbit anti-ERG (Cell Signaling Technologies 97249); rat anti-PECAM1/CD31 (BD Biosciences 553370); mouse anti-CLDN5, Alexa Fluor 488 conjugate (Invitrogen 352588); mouse anti-CLDN5 (Invitrogen 35–2500); rabbit anti-ZO1 (Invitrogen 40–2200); rabbit anti-LEF1 rabbit (Cell Signaling Technologies 2230); rabbit anti-LEF1, Alexa Fluor 647 conjugate (Cell Signaling Technologies 14022); rabbit anti-Occludin (Invitrogen 406100); goat anti-VEGFR2/KDR (R&D Systems AF644-SP); sheep anti-FOXP2 (R&D Systems AF5647-SP); rabbit anti-SATB2 (Abcam ab92446); rat anti-IL-6 (Biolegend 504501); rabbit anti-COL14A1 (Novus Biologicals NBP2-15940); chicken anti-GFP (Abcam Ab13970), rat anti-HA (Proteintech 7c9); rabbit anti-HA (homemade); goat anti-E-cadherin (R&D Systems AF748); rabbit anti-E-cadherin (Cell Signaling Technologies 3195); rabbit anti-AIFM3 (Novus Biologicals NBP1-76889); rabbit anti-COL25A1 (G-Biosciences ITT1021); goat anti-IGSF8 (R&D systems AF3117-SP); rabbit anti-NNAT (Abcam ab27266); mouse anti-GAPDH (Cell Signaling Technologies 97166 S); rabbit anti-GAPDH (Cell Signaling Technologies 5174 S); Streptavidin, Alexa Fluor 488 conjugate (Invitrogen S11223); Streptavidin, Alexa Fluor 647 conjugate (Invitrogen S32357). .. Alexa-Fluor-conjugated secondary antibodies were from Invitrogen.
Article Title: Bacterial meningitis in the early postnatal mouse studied at single-cell resolution
Article Snippet: .. The following antibodies were used for tissue immunohistochemistry and immunoblotting: goat antiCD45 (R&D Systems AF114- SP); rat anti- CD206/MRC1 (Bio- Rad MCA2235T); goat anti- CD206 (R&D Systems AP2535); rat anti- LYVE1 (Thermo Fisher/eBioscience 14- 0443- 82); rat anti- PU.1/Spi- 1 (Novus Biologicals MAB7124); rat anti- CD180/RP105 antibody, PE (eBioscience 12- 1801- 81); goat anti- S100A8 (R&D Systems AF3059); rabbit anti- ERG (Cell Signaling Technologies 97249); rat anti- PECAM1/CD31 (BD Biosciences 553370); mouse anti- CLDN5, Alexa Fluor 488 conjugate (Invitrogen 352588); mouse anti- CLDN5 (Invitrogen 35–2500); rabbit anti- ZO1 (Invitrogen 40–2200); rabbit anti- LEF1 rabbit (Cell Signaling Technologies 2230); rabbit anti- LEF1, Alexa Fluor 647 conjugate (Cell Signaling Technologies 14022); rabbit anti- Occludin (Invitrogen 406100); goat anti- VEGFR2/KDR (R&D Systems AF644- SP); sheep anti- FOXP2 (R&D Systems AF5647- SP); rabbit anti- SATB2 (Abcam ab92446); rat anti- IL- 6 (Biolegend 504501); rabbit anti- COL14A1 (Novus Biologicals NBP2- 15940); chicken anti- GFP (Abcam Ab13970), rat anti- HA (Proteintech 7c9); rabbit anti- HA (homemade); goat anti- E- cadherin (R&D Systems AF748); rabbit anti- E- cadherin (Cell Signaling Technologies 3195); rabbit anti- AIFM3 (Novus Biologicals NBP1- 76889); rabbit anti- COL25A1 (G- Biosciences ITT1021); goat anti- IGSF8 (R&D systems AF3117- SP); rabbit anti- NNAT (Abcam ab27266); mouse anti- GAPDH (Cell Signaling Technologies 97166 S); rabbit anti- GAPDH (Cell Signaling Technologies 5174 S); Streptavidin, Alexa Fluor 488 conjugate (Invitrogen S11223); Streptavidin, Alexa Fluor 647 conjugate (Invitrogen S32357). .. AlexaFluor- conjugated secondary antibodies were from Invitrogen.
Western Blot:Article Title: Bacterial meningitis in the early postnatal mouse studied at single-cell resolution
Article Snippet: .. The following antibodies were used for tissue immunohistochemistry and immunoblotting: goat anti-CD45 (R&D Systems AF114-SP); rat anti-CD206/MRC1 (Bio-Rad MCA2235T); goat anti-CD206 (R&D Systems AP2535); rat anti-LYVE1 (Thermo Fisher/eBioscience 14-0443-82); rat anti-PU.1/Spi-1 (Novus Biologicals MAB7124); rat anti-CD180/RP105 antibody, PE (eBioscience 12-1801-81); goat anti-S100A8 (R&D Systems AF3059); rabbit anti-ERG (Cell Signaling Technologies 97249); rat anti-PECAM1/CD31 (BD Biosciences 553370); mouse anti-CLDN5, Alexa Fluor 488 conjugate (Invitrogen 352588); mouse anti-CLDN5 (Invitrogen 35-2500); rabbit anti-ZO1 (Invitrogen 40-2200); rabbit anti-LEF1 rabbit (Cell Signaling Technologies 2230); rabbit anti-LEF1, Alexa Fluor 647 conjugate (Cell Signaling Technologies 14022); rabbit anti-Occludin (Invitrogen 406100); goat anti-VEGFR2/KDR (R&D Systems AF644-SP); sheep anti-FOXP2 (R&D Systems AF5647-SP); rabbit anti-SATB2 (Abcam ab92446); rat anti-IL-6 (Biolegend 504501); rabbit anti-COL14A1 (Novus Biologicals NBP2-15940); chicken anti-GFP (Abcam Ab13970), rat anti-HA (Proteintech 7c9); rabbit anti-HA (homemade); goat anti-E-cadherin (R&D Systems AF748); rabbit anti-E-cadherin (Cell Signaling Technologies 3195); rabbit anti-AIFM3 (Novus Biologicals NBP1-76889); rabbit anti-COL25A1 (G-Biosciences ITT1021); goat anti-IGSF8 (R&D systems AF3117-SP); rabbit anti-NNAT (Abcam ab27266); mouse anti-GAPDH (Cell Signaling Technologies 97166S); rabbit anti-GAPDH (Cell Signaling Technologies 5174S); Streptavidin, Alexa Fluor 488 conjugate (Invitrogen S11223); Streptavidin, Alexa Fluor 647 conjugate (Invitrogen S32357). .. Alexa-Fluor-conjugated secondary antibodies were from Invitrogen.
Article Title: Bacterial meningitis in the early postnatal mouse studied at single-cell resolution
Article Snippet: .. The following antibodies were used for tissue immunohistochemistry and immunoblotting: goat anti-CD45 (R&D Systems AF114-SP); rat anti-CD206/MRC1 (Bio-Rad MCA2235T); goat anti-CD206 (R&D Systems AP2535); rat anti-LYVE1 (Thermo Fisher/eBioscience 14-0443-82); rat anti-PU.1/Spi-1 (Novus Biologicals MAB7124); rat anti-CD180/RP105 antibody, PE (eBioscience 12-1801-81); goat anti-S100A8 (R&D Systems AF3059); rabbit anti-ERG (Cell Signaling Technologies 97249); rat anti-PECAM1/CD31 (BD Biosciences 553370); mouse anti-CLDN5, Alexa Fluor 488 conjugate (Invitrogen 352588); mouse anti-CLDN5 (Invitrogen 35–2500); rabbit anti-ZO1 (Invitrogen 40–2200); rabbit anti-LEF1 rabbit (Cell Signaling Technologies 2230); rabbit anti-LEF1, Alexa Fluor 647 conjugate (Cell Signaling Technologies 14022); rabbit anti-Occludin (Invitrogen 406100); goat anti-VEGFR2/KDR (R&D Systems AF644-SP); sheep anti-FOXP2 (R&D Systems AF5647-SP); rabbit anti-SATB2 (Abcam ab92446); rat anti-IL-6 (Biolegend 504501); rabbit anti-COL14A1 (Novus Biologicals NBP2-15940); chicken anti-GFP (Abcam Ab13970), rat anti-HA (Proteintech 7c9); rabbit anti-HA (homemade); goat anti-E-cadherin (R&D Systems AF748); rabbit anti-E-cadherin (Cell Signaling Technologies 3195); rabbit anti-AIFM3 (Novus Biologicals NBP1-76889); rabbit anti-COL25A1 (G-Biosciences ITT1021); goat anti-IGSF8 (R&D systems AF3117-SP); rabbit anti-NNAT (Abcam ab27266); mouse anti-GAPDH (Cell Signaling Technologies 97166 S); rabbit anti-GAPDH (Cell Signaling Technologies 5174 S); Streptavidin, Alexa Fluor 488 conjugate (Invitrogen S11223); Streptavidin, Alexa Fluor 647 conjugate (Invitrogen S32357). .. Alexa-Fluor-conjugated secondary antibodies were from Invitrogen.
Article Title: Bacterial meningitis in the early postnatal mouse studied at single-cell resolution
Article Snippet: .. The following antibodies were used for tissue immunohistochemistry and immunoblotting: goat antiCD45 (R&D Systems AF114- SP); rat anti- CD206/MRC1 (Bio- Rad MCA2235T); goat anti- CD206 (R&D Systems AP2535); rat anti- LYVE1 (Thermo Fisher/eBioscience 14- 0443- 82); rat anti- PU.1/Spi- 1 (Novus Biologicals MAB7124); rat anti- CD180/RP105 antibody, PE (eBioscience 12- 1801- 81); goat anti- S100A8 (R&D Systems AF3059); rabbit anti- ERG (Cell Signaling Technologies 97249); rat anti- PECAM1/CD31 (BD Biosciences 553370); mouse anti- CLDN5, Alexa Fluor 488 conjugate (Invitrogen 352588); mouse anti- CLDN5 (Invitrogen 35–2500); rabbit anti- ZO1 (Invitrogen 40–2200); rabbit anti- LEF1 rabbit (Cell Signaling Technologies 2230); rabbit anti- LEF1, Alexa Fluor 647 conjugate (Cell Signaling Technologies 14022); rabbit anti- Occludin (Invitrogen 406100); goat anti- VEGFR2/KDR (R&D Systems AF644- SP); sheep anti- FOXP2 (R&D Systems AF5647- SP); rabbit anti- SATB2 (Abcam ab92446); rat anti- IL- 6 (Biolegend 504501); rabbit anti- COL14A1 (Novus Biologicals NBP2- 15940); chicken anti- GFP (Abcam Ab13970), rat anti- HA (Proteintech 7c9); rabbit anti- HA (homemade); goat anti- E- cadherin (R&D Systems AF748); rabbit anti- E- cadherin (Cell Signaling Technologies 3195); rabbit anti- AIFM3 (Novus Biologicals NBP1- 76889); rabbit anti- COL25A1 (G- Biosciences ITT1021); goat anti- IGSF8 (R&D systems AF3117- SP); rabbit anti- NNAT (Abcam ab27266); mouse anti- GAPDH (Cell Signaling Technologies 97166 S); rabbit anti- GAPDH (Cell Signaling Technologies 5174 S); Streptavidin, Alexa Fluor 488 conjugate (Invitrogen S11223); Streptavidin, Alexa Fluor 647 conjugate (Invitrogen S32357). .. AlexaFluor- conjugated secondary antibodies were from Invitrogen.
Chromatin Immunoprecipitation:Article Title: Dynamic fibroblast-immune interactions shape recovery after brain injury.
Article Snippet: .. Materials & experimental systems n/a Involved in the study Antibodies Eukaryotic cell lines Palaeontology and archaeology Animals and other organisms Clinical data Dual use research of concern Plants Methods n/a Involved in the study ChIP-seq Flow cytometry MRI-based neuroimaging Antibodies Antibodies used Primary antibodies used for murine imaging include chicken anti-GFP (Aves Labs GFP-1020, 1:200), rabbit anti-dsRed (Takara 632496, 1:300), chicken anti-GFAP (Invitrogen PA1-10004, 1:200 or 1:500), rat anti-GFAP (2.2B10, Invitrogen 13-0300, 1:200), rat anti-ER-TR7 (Novus Biologicals NB100-64932, 1:200), rabbit anti-aSMA (Abcam ab5694, 1:300), rat anti-CD31 (MEC13.3, Biolegend 102514, 1:200), goat anti-Desmin (GenWay Biotech GWB-EV0472, 1:200), rat anti-PDGFRb (APB5, Invitrogen 14-1402-82, 1:500), rabbit antiNG2 (Millipore Sigma ab5320, 1:200), goat anti-Decorin (Novus Biologicals AF1060, 1:200), goat anti-Collagen 1 (Southern Biotech 1310-01, 1:500), rabbit anti-Collagen 6a1 (Novus Biologicals NB120-6588, 1:200 or 1:500), rat anti-Periostin (345613, Novus Biologicals MAB3548, 1:200), rat anti-ICAM1 (YN1/1.7.4, Biolegend 116110, 1:200), syrian hamster-anti-CD3e (500A2, BD Biosciences 553238, 1:200), goat anti-S100A8 (R&D Systems AF3059, 1:200), chicken anti-NeuN (Millipore Sigma ABN91, 1:200), rabbit anti-Iba1 (Aif3, Fujifilm Wako 019-19741, 1:200-1:1000), mouse anti-FGF13 (N235/22, Invitrogen MA5-27705, 1:100), goat anti-CD80 (R&D Systems AF740, 1:200), goat anti-ALPL (Novus Biologicals AF2910, 1:50), rabbit anti-LAMA1 (EPR27258-37, Abcam ab307542, 1:200), rabbit anti-SEMA3C (Invitrogen PA5-103168, 1:100), rabbit anti-CDH18 (Invitrogen PA5-112902, 1:50), rabbit anti-ALDH1A2 (Novus Biologicals NBP2-92915, 1:200), goat anti-SOX10 (R&D Systems AF2864, 1:300), rabbit anti-ASPA (Genetex GTX113389, 1:1000), mouse anti-E-Cadherin (Clone 36, BD Biosciences 610181), rat anti-I-A/I-E (MHCII, M5/114.15.2, eBioscience 14-5321-82), mouse anti-Ly76 (TER119, Biolegend 116232, 1:200), goat anti-mouse IgM (Invitrogen 31172, 1:200), and rabbit anti cleaved Caspase 3 (Cell Signaling Technology 9661T, 1:400; Cell Signaling Technology 9991, Histowiz). ..
Flow Cytometry:Article Title: Dynamic fibroblast-immune interactions shape recovery after brain injury.
Article Snippet: .. Materials & experimental systems n/a Involved in the study Antibodies Eukaryotic cell lines Palaeontology and archaeology Animals and other organisms Clinical data Dual use research of concern Plants Methods n/a Involved in the study ChIP-seq Flow cytometry MRI-based neuroimaging Antibodies Antibodies used Primary antibodies used for murine imaging include chicken anti-GFP (Aves Labs GFP-1020, 1:200), rabbit anti-dsRed (Takara 632496, 1:300), chicken anti-GFAP (Invitrogen PA1-10004, 1:200 or 1:500), rat anti-GFAP (2.2B10, Invitrogen 13-0300, 1:200), rat anti-ER-TR7 (Novus Biologicals NB100-64932, 1:200), rabbit anti-aSMA (Abcam ab5694, 1:300), rat anti-CD31 (MEC13.3, Biolegend 102514, 1:200), goat anti-Desmin (GenWay Biotech GWB-EV0472, 1:200), rat anti-PDGFRb (APB5, Invitrogen 14-1402-82, 1:500), rabbit antiNG2 (Millipore Sigma ab5320, 1:200), goat anti-Decorin (Novus Biologicals AF1060, 1:200), goat anti-Collagen 1 (Southern Biotech 1310-01, 1:500), rabbit anti-Collagen 6a1 (Novus Biologicals NB120-6588, 1:200 or 1:500), rat anti-Periostin (345613, Novus Biologicals MAB3548, 1:200), rat anti-ICAM1 (YN1/1.7.4, Biolegend 116110, 1:200), syrian hamster-anti-CD3e (500A2, BD Biosciences 553238, 1:200), goat anti-S100A8 (R&D Systems AF3059, 1:200), chicken anti-NeuN (Millipore Sigma ABN91, 1:200), rabbit anti-Iba1 (Aif3, Fujifilm Wako 019-19741, 1:200-1:1000), mouse anti-FGF13 (N235/22, Invitrogen MA5-27705, 1:100), goat anti-CD80 (R&D Systems AF740, 1:200), goat anti-ALPL (Novus Biologicals AF2910, 1:50), rabbit anti-LAMA1 (EPR27258-37, Abcam ab307542, 1:200), rabbit anti-SEMA3C (Invitrogen PA5-103168, 1:100), rabbit anti-CDH18 (Invitrogen PA5-112902, 1:50), rabbit anti-ALDH1A2 (Novus Biologicals NBP2-92915, 1:200), goat anti-SOX10 (R&D Systems AF2864, 1:300), rabbit anti-ASPA (Genetex GTX113389, 1:1000), mouse anti-E-Cadherin (Clone 36, BD Biosciences 610181), rat anti-I-A/I-E (MHCII, M5/114.15.2, eBioscience 14-5321-82), mouse anti-Ly76 (TER119, Biolegend 116232, 1:200), goat anti-mouse IgM (Invitrogen 31172, 1:200), and rabbit anti cleaved Caspase 3 (Cell Signaling Technology 9661T, 1:400; Cell Signaling Technology 9991, Histowiz). ..
Magnetic Resonance Imaging:Article Title: Dynamic fibroblast-immune interactions shape recovery after brain injury.
Article Snippet: .. Materials & experimental systems n/a Involved in the study Antibodies Eukaryotic cell lines Palaeontology and archaeology Animals and other organisms Clinical data Dual use research of concern Plants Methods n/a Involved in the study ChIP-seq Flow cytometry MRI-based neuroimaging Antibodies Antibodies used Primary antibodies used for murine imaging include chicken anti-GFP (Aves Labs GFP-1020, 1:200), rabbit anti-dsRed (Takara 632496, 1:300), chicken anti-GFAP (Invitrogen PA1-10004, 1:200 or 1:500), rat anti-GFAP (2.2B10, Invitrogen 13-0300, 1:200), rat anti-ER-TR7 (Novus Biologicals NB100-64932, 1:200), rabbit anti-aSMA (Abcam ab5694, 1:300), rat anti-CD31 (MEC13.3, Biolegend 102514, 1:200), goat anti-Desmin (GenWay Biotech GWB-EV0472, 1:200), rat anti-PDGFRb (APB5, Invitrogen 14-1402-82, 1:500), rabbit antiNG2 (Millipore Sigma ab5320, 1:200), goat anti-Decorin (Novus Biologicals AF1060, 1:200), goat anti-Collagen 1 (Southern Biotech 1310-01, 1:500), rabbit anti-Collagen 6a1 (Novus Biologicals NB120-6588, 1:200 or 1:500), rat anti-Periostin (345613, Novus Biologicals MAB3548, 1:200), rat anti-ICAM1 (YN1/1.7.4, Biolegend 116110, 1:200), syrian hamster-anti-CD3e (500A2, BD Biosciences 553238, 1:200), goat anti-S100A8 (R&D Systems AF3059, 1:200), chicken anti-NeuN (Millipore Sigma ABN91, 1:200), rabbit anti-Iba1 (Aif3, Fujifilm Wako 019-19741, 1:200-1:1000), mouse anti-FGF13 (N235/22, Invitrogen MA5-27705, 1:100), goat anti-CD80 (R&D Systems AF740, 1:200), goat anti-ALPL (Novus Biologicals AF2910, 1:50), rabbit anti-LAMA1 (EPR27258-37, Abcam ab307542, 1:200), rabbit anti-SEMA3C (Invitrogen PA5-103168, 1:100), rabbit anti-CDH18 (Invitrogen PA5-112902, 1:50), rabbit anti-ALDH1A2 (Novus Biologicals NBP2-92915, 1:200), goat anti-SOX10 (R&D Systems AF2864, 1:300), rabbit anti-ASPA (Genetex GTX113389, 1:1000), mouse anti-E-Cadherin (Clone 36, BD Biosciences 610181), rat anti-I-A/I-E (MHCII, M5/114.15.2, eBioscience 14-5321-82), mouse anti-Ly76 (TER119, Biolegend 116232, 1:200), goat anti-mouse IgM (Invitrogen 31172, 1:200), and rabbit anti cleaved Caspase 3 (Cell Signaling Technology 9661T, 1:400; Cell Signaling Technology 9991, Histowiz). ..
|
R&D Systems is a verified supplier 
R&D Systems manufactures this product 
